Influence of adult Schwann cells and olfactory ensheathing glia on axontarget cell interactions in the CNS a comparative analysis using a retinotectal cograft model
ثبت نشده
چکیده
We used an in vivo transplant approach to examine how adult Schwann cells and olfactory ensheathing glia (OEG) influence the specificity of axon–target cell interactions when they are introduced into the CNS. Populations of either Schwann cells or OEG were mixed with dissociated fetal tectal cells (presumptive superior colliculus) and, after re-aggregation, pieces were grafted onto newborn rat superior colliculus. Both glial types were prelabeled with lentiviral vectors encoding green fluorescent protein. Grafts rapidly established fiber connections with the host and retinal projections into co-grafts were assessed 6–56 days post-transplantation by injecting cholera toxin B into host eyes. In control rats that received pure dissociated– reaggregated tectal grafts, retinal ganglion cell (RGC) axons selectively innervated defined target areas, corresponding to the retinorecipient layer in normal superior colliculus. The pattern of RGC axon ingrowth into OEG containing co-grafts was similar to that in control grafts. However, in Schwann cell co-grafts there was reduced host retinal input into presumptive target areas and many RGC axons were scattered throughout the neuropil. Given that OEG in co-grafts had minimal impact on axon–target cell recognition, OEG might be an appropriate cell type for direct transplantation into injured neuropil when attempting to stimulate specific pathway reconstruction.
منابع مشابه
Influence of adult Schwann cells and olfactory ensheathing glia on axon-target cell interactions in the CNS: a comparative analysis using a retinotectal co-graft model
متن کامل
Influence of adult Schwann cells and olfactory ensheathing glia on axon-target cell interactions in the CNS: a comparative analysis using a retinotectal co-graft model
متن کامل
Influence of adult Schwann cells and olfactory ensheathing glia on axon--target cell interactions in the CNS: a comparative analysis using a retinotectal co-graft model.
We used an in vivo transplant approach to examine how adult Schwann cells and olfactory ensheathing glia OEG influence the specificity of axon-target cell interactions when they are introduced into the CNS. Populations of either Schwann cells or OEG were mixed with dissociated fetal tectal cells presumptive superior colliculus and, after reaggregation, pieces were grafted onto newborn rat super...
متن کاملProtective effect of adult olfactory ensheathing cells against 6-OHDA toxicity in PC-12 cells
Olfactory ensheathing cells (OEC) have been successfully used to stimulate the growth of injured fibers and to promote functional recovery within the injured adult CNS. These cells exhibit functional properties, which are known to be involved in axonal elongation. OEC express high level of growth factors including NGF, GDNF, BDNF and NT-3, which are known to play an important role in nerve rege...
متن کاملComparative gene expression profiling of olfactory ensheathing glia and Schwann cells indicates distinct tissue repair characteristics of olfactory ensheathing glia.
Olfactory ensheathing glia (OEG) are a specialized type of glia that support the growth of primary olfactory axons from the neuroepithelium in the nasal cavity to the brain. Transplantation of OEG in the injured spinal cord promotes sprouting of injured axons and results in reduced cavity formation, enhanced axonal and tissue sparing, remyelination, and angiogenesis. Gene expression analysis ma...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2008